suredbyrt-pcr,andthediameteroftheextracellularcoatalmostd

isaearcompletely.seeandreversehas-2showednoeffect.
4.theimpactofinflammationonhyaluronansynthesesandaemblyinhumanperitonealmesothelialcells(hpmcs)
hpmcswereculturedandstimulatedwithl,il-1bortnf-a.differenthasynthesizinggeneshas-2(whichsynthesizehighmolecularweighthyaluronan),andhas3(whichsynthesizelowmolecularweighthyaluronan),weredetectedbyrt-pcr.thepericellularhyaluronancoatwasalsovisualizedbyparticleexclusionaay.theconcentrationofhyaluronaniculturedmediumwasmeasuredbyhyaluronanradio-immunoaaykits.
resultl,il-1bortnf-aallstimulatedhas-2andhas-3mrnaexpreion.theeffectsoftheseinflammatorymediatorsonhas3expreionwasmuchhigherascomparedtotheireffectsonhas-2expreion,eeciallywiththestimulationofil-1bwhichincreasedthehas-3mrnaby19times.however,thesizesofthepericelluarhyaluronancoatinhpmcsdidnotchangesignificantlyafterl,il-1bortnf-astimulation.theconcentrationofhyaluronanininflammationmediatorsgrouweresignificantlyhigherthanthecontrolgroup.
5.theeffectofosmoticagentsonhyaluronansynthasesexpreioninhumanperitonealmesothelialcells
culturedhpmcswerestimulatedwith90mmol/lglucose(hg),30mmol/lglucose(lg),5polyglucose(pg)and90mmol/lmaitol(mol).extracellularcellcoatwasoervedusingparticleexclusionaay,andtheexpreionofhyaluronansynthase2and3(has-2andhas-3)mrnainhpmcswasmeasuredbyreversetracription-polymerasechainreaction(rt-pcr).theconcentrationofhyaluronaniculturedmediumwasmeasuredbyhyaluronanradio-immunoaaykits.

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